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Dairy Antibiotics Spore High-throughput Test Kit

DASH (Dairy Antibiotics Spore High-throughput) Test Kit is applicable to qualitative analysis of antibiotic residues in raw milk (including cattle, sheep, and goats).

Flyer – DASH Test Kit. pdf

Brochure-Dairy Test. PDF

Meizheng Bio-Tech Company Overview. PDF

ManufacturerMeizheng
Order No.WC0006
AnalyteAntibiotic residues
Sample TypeRaw milk (including cattle, sheep, and goats).
MethodologyMicrobial inhibition
Packaging96 Tests
Storage4-8℃
Shelf lifeThe validity for this kit is 12 months
Lead timeShipped within 5-12 working days.

Principle of Test

This kit adopts the principle of microbial inhibition. The microwell plate is filled with a medium containing Geobacillus stearothermophilus spores and the pH indicator. When cultured at 65℃, the spores germinate, the bacteria grow, and then metabolize to produce acid. The pH value decreases and the indicator changes from the original purple to yellow. If the antibiotics exist in the added milk and dairy products, the antibiotics will inhibit bacterial growth, thus no acid is produced, the indicator does not change the color, and the original purple is maintained; if the antibiotics do not exist in the added milk and dairy products or the concentration of antibiotics is below the detection limit, the spores germinate, metabolize and product acid, and the medium turns yellow.

Detection Limit

AntibioticDetection Limit (μg/L)
Beta-lactams
Penicillin G2
Ampicillin2-3
Amoxicillin2-3
Nafcillin5
Cloxacillin10-15
Oxacillin6
Dicloxacillin10-15
Cefacetrile20
Cefalexin45
Cefalonium5-6
Cefoperazone30
Ceftiofur50
Cefquinome75
Cefapirin2.5-5
Cefazolin10-15
Tetracyclines
Chlortetracycline200
Doxycycline75-100
Oxytetracycline100-150
Tetracycline100-150
Aminoglycosides
Gentamicin100
Kanamycin2500
Neomycin200-300
Spectinomycin>300
Streptomycin1100-1500
Dihydrostreptomycin700-1000
Sulfonamides
Sulfamethazine50-100
Sulfadimethoxine25-50
Sulfamerazine100
Sulfathiazole40
Sulfadiazine50
Sulfadoxine80-120
Sulfamethoxazole<25
Sulfamethoxpyridazine50-100
Sulfisoxazole50
Sulfachloropyridazine25-50
Sulfaphenazole25-50
Sulfamethoxydiazine50-100
Sulfabenzamide100
Sulfaclozine100
Sulfapyrazole50
Sulfamethoxypyrazine25-50
Sulfisomidine50-100
Sulfamonomethoxine50
Sulfapyridine100
Sulfaquinoxaline50
Sulfamethazole100
Sulfamethizole50-100
Macrolides
Erythromycin100-150
Spiramycin400-800
Tilmcosin100
Tylosin40-80
Quinolones
Ciprofloxacin1000-1500
Danofloxacin1000-2000
Marbofloxacin>1000
Various
Chloramphenicol2500
Rifaximin60-100
Pirlimycin200
Thiamphenicol>1000
Trimethoprim100
Lincomycin300-500

Reagents and Materials Supplied

Detachable microwell plate3 pieces

(96 independent tests each piece)

Cover film (Aluminum foil)3
User manual1
Colorimetric card1

Materials required but not supplied

—- Stable 65°C heating equipment (water bath or dry heater)

—- Single channel micropipette (2.5-100µL)

—- Negative control: milk without antibiotics

—- Positive control: milk containing 10mg/L Penicillin G or 10mg/L Ampicillin

Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.

 

Quantity

1-10, 11-100, 101-1000, 1001-10000

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